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Anti-Human IL-1 Receptor Type I Secondary Antibody
Rabbit Polyclonal, Unconjugated
- 产品详情
- 实验流程
- 背景知识
| Description | Anti-Human IL-1 Receptor Type I (IL-1RI) (RABBIT) Antibody |
|---|---|
| Host | Rabbit |
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human |
| Clonality | Polyclonal |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | Antiserum |
| Buffer | 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | IL-1 Receptor Type I Antibody was prepared by repeated immunizations with recombinant human IL-1R type I. |
| Stabilizer | None |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Dry Ice |
|---|---|
| Application Note | This antiserum against anti-Human IL-1 Receptor Type I (IL-1RI) has been tested for use in ELISA, radioimmunoassays and immunoprecipitation. Reactivity in other immunoassays is unknown.This product has been assayed for the ability to immunoprecipitate antigen. A dilution range of 1:400 to 1:800 is suggested for this immunoassay. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. This product has been assayed by ELISA against soluble (extracellular) IL-1RI using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:1,000 to 1:2000 is suggested for this product. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8,000 is suggested for this immunoassay. |
| Purity | This antiserum has been heated to 56°C for 30 minutes. The antiserum is directed against the extracellular domain of the human IL-1R type I, but precipitates the intact receptor expressed on human cells. In general, this antibody also detects primate IL-1RI. The antibody does not recognize human IL-1, IL-1Ra or IL-1R type II. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type I (also called soluble IL-1R type I or ''decoy'' receptor) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid. |
| Storage Condition | Store IL-1 Receptor Type I Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
IL-1 Receptor Type I is a cytokine receptor which binds interleukin 1. Anti-IL-1 Receptor Type I antibody is ideal for researchers interested in Immunology, Signal Transduction and Cancer.
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