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Anti-Bovine IgG (H&L) (Peroxidase Conjugated) Secondary Antibody

Rabbit Polyclonal, Peroxidase (Horseradish)

     
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Product Information
Description Anti-BOVINE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated
Host Rabbit
Conjugate Peroxidase (Horseradish)
Target Species Bovine
Clonality Polyclonal
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Bovine IgG whole molecule
Reconstitution Volume 2.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Additional Information
Shipping Condition Ambient
Application Note Anti-BOVINE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:17,000 to 1:70,000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:17,000 to 1:70,000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:17,000 to 1:70,000 of the reconstitution concentration is suggested for this product.
Purity Anti-BOVINE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Bovine IgG and Bovine Serum.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

BACKGROUND

Anti-BOVINE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated generated in goat detects specifically bovine IgG whole molecule. Anti-Bovine IgG antibody is ideal for investigators involved in Serum Protein Component Protein research.

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