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Anti-Chicken IgG (H&L) (Peroxidase Conjugated) Secondary Antibody

Rabbit Polyclonal, Peroxidase (Horseradish)

     
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Product Information
Description Anti-CHICKEN IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated
Host Rabbit
Conjugate Peroxidase (Horseradish)
Target Species Chicken
Clonality Polyclonal
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Chicken IgG whole molecule
Reconstitution Volume 2.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative None
Additional Information
Shipping Condition Ambient
Application Note This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:8,000 to 1:46,000 of the reconstitution concentration is suggested for this product. 
Purity This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Chicken IgG and Chicken Serum.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Anti-Chicken IgG antibody generated in rabbit detects specifically chicken IgG. This secondary antibody anti-Chicken is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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