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RABBIT IgG (BULK ORDER)

     
  • 1 - RABBIT IgG (BULK ORDER) ASR3580
    SDS-Page of Rabbit IgG. Lane 1: Rabbit IgG - Non-Reduced. Lane 2: Rabbit IgG - Reduced. Load: 1.0 ug per lane. Predicted/Observed Size: Non-reduced - 130 kDa, Reduced - 55 and 28 kDa for Rabbit IgG. Other Band(s): None.
  • 1 - RABBIT IgG (BULK ORDER) ASR3580
    SDS-PAGE of Rabbit IgG Whole Molecule. Lane 1: Non-reduced Rabbit IgG Whole Molecule. Lane 2: 5 µL OPAL Pre-stained Marker (MB-210-0500). Lane 3: Reduced Rabbit IgG Whole Molecule. Load: 1 µg per lane. Predicted/Observed size: Non-reduced at 150-170 kDa , Reduced at 55, 25 kDa.
  • 1 - RABBIT IgG (BULK ORDER) ASR3580
    SDS-PAGE of Rabbit IgG Whole Molecule (BULK ORDER) (p/n ASR3580). Lane M: 3 µL Opal Prestained Marker (p/n MB-210-0500). Lane 1: Reduced Rabbit IgG Whole Molecule (BULK ORDER) (p/n ASR3580). Lane 2: Reduced Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n 011-0103). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n 011-0107). Load: 1 µg for F(ab) and F(c); 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
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Product Information
Description RABBIT IgG whole molecule (BULK ORDER)
Conjugate Unconjugated
Physical State Lyophilized
Host Isotype IgG
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Species of Origin Rabbit
Reconstitution Volume 5.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Application Note Rabbit IgG whole molecule can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Purity Rabbit IgG whole molecular was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Rabbit IgG whole molecular was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG and anti-Rabbit Serum.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Rabbit IgG whole molecule is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-afinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

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