癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
DHFR Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant DHFR.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E |
|---|---|
| Primary Accession | P00374 |
| Other Accession | BC003584 |
| Reactivity | Human, Rat |
| Host | mouse |
| Clonality | monoclonal |
| Isotype | IgG2a Kappa |
| Clone Names | 2B10 |
| Calculated MW | 21453 Da |
| Gene ID | 1719 |
|---|---|
| Other Names | Dihydrofolate reductase, DHFR |
| Target/Specificity | DHFR (AAH03584, 88 a.a. ~ 187 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IF~~1:50~200 E~~N/A |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | DHFR Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.
REFERENCES
1.Influence of Reduced Folate Carrier and Dihydrofolate Reductase Genes on Methotrexate-Induced Cytotoxicity.Yoon SA, Choi JR, Kim JO, Shin JY, Zhang X, Kang JH.Cancer Res Treat. 2010 Sep;42(3):163-71. Epub 2010 Sep 30.2.Telomere capping in non-dividing yeast cells requires Yku and Rap1.Vodenicharov MD, Laterreur N, Wellinger RJ.EMBO J. 2010 Jul 13. [Epub ahead of print]3.Anticancer drug encapsulated in inorganic lattice can overcome drug resistance.Choi SJ, Choi GE, Oh JM, Oh YJ, Park MC, Choy JH.J. Mater. Chem., 2010, 20, 9463-94694.Deficient BH4 production via de novo and salvage pathways regulates NO responses to cytokines in adult cardiac myocytes.Ionova IA, Vasquez-Vivar J, Whitsett J, Herrnreiter A, Medhora M, Cooley BC, Pieper GM.Am J Physiol Heart Circ Physiol. 2008 Nov;295(5):H2178-87. Epub 2008 Oct 3.
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