癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
E2F6 Antibody (monoclonal) (M02)
Mouse monoclonal antibody raised against a full length recombinant E2F6.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E |
|---|---|
| Primary Accession | O75461 |
| Other Accession | BC008348 |
| Reactivity | Human |
| Host | mouse |
| Clonality | monoclonal |
| Isotype | IgG2a kappa |
| Clone Names | 1F7-A9 |
| Calculated MW | 31844 Da |
| Gene ID | 1876 |
|---|---|
| Other Names | Transcription factor E2F6, E2F-6, E2F6 |
| Target/Specificity | E2F6 (AAH08348, 1 a.a. ~ 281 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IF~~1:50~200 E~~N/A |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | E2F6 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms.
REFERENCES
Cell cycle genes and ovarian cancer susceptibility: a tagSNP analysis. Cunningham JM, et al. Br J Cancer, 2009 Oct 20. PMID 19738611.E2F6 inhibits cobalt chloride-mimetic hypoxia-induced apoptosis through E2F1. Yang WW, et al. Mol Biol Cell, 2008 Sep. PMID 18562691.A comprehensive ChIP-chip analysis of E2F1, E2F4, and E2F6 in normal and tumor cells reveals interchangeable roles of E2F family members. Xu X, et al. Genome Res, 2007 Nov. PMID 17908821.E2F-6 suppresses growth-associated apoptosis of human hematopoietic progenitor cells by counteracting proapoptotic activity of E2F-1. Kikuchi J, et al. Stem Cells, 2007 Oct. PMID 17600109.The histone H3K4 demethylase SMCX links REST target genes to X-linked mental retardation. Tahiliani M, et al. Nature, 2007 May 31. PMID 17468742.
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