癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
E4F1 Antibody (monoclonal) (M03)
Mouse monoclonal antibody raised against a partial recombinant E4F1.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF, E |
|---|---|
| Primary Accession | Q66K89 |
| Other Accession | NM_004424 |
| Reactivity | Human |
| Host | mouse |
| Clonality | monoclonal |
| Isotype | IgG2a Kappa |
| Clone Names | 4B10 |
| Calculated MW | 83496 Da |
| Gene ID | 1877 |
|---|---|
| Other Names | Transcription factor E4F1, 632-, E4F transcription factor 1, Putative E3 ubiquitin-protein ligase E4F1, Transcription factor E4F, p120E4F, p50E4F, E4F1, E4F |
| Target/Specificity | E4F1 (NP_004415.2, 685 a.a. ~ 784 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IF~~1:50~200 E~~N/A |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | E4F1 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence.
REFERENCES
The role of LANP and ataxin 1 in E4F-mediated transcriptional repression. Cvetanovic M, et al. EMBO Rep, 2007 Jul. PMID 17557114.E4F1 is an atypical ubiquitin ligase that modulates p53 effector functions independently of degradation. Le Cam L, et al. Cell, 2006 Nov 17. PMID 17110336.The LIM-only protein FHL2 is a negative regulator of E4F1. Paul C, et al. Oncogene, 2006 Sep 7. PMID 16652157.Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.E4F1, a novel estrogen-responsive gene in possible atheroprotection, revealed by microarray analysis. Nakamura Y, et al. Am J Pathol, 2004 Dec. PMID 15579445.
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