癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ETF1 Antibody (monoclonal) (M02)
Mouse monoclonal antibody raised against a partial recombinant ETF1.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | P62495 |
| Other Accession | NM_004730 |
| Reactivity | Human |
| Host | mouse |
| Clonality | monoclonal |
| Isotype | IgG2b Kappa |
| Clone Names | 2H4 |
| Other Names | Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, eRF1, Protein Cl1, TB3-1, ETF1, ERF1, RF1, SUP45L1 |
|---|---|
| Target/Specificity | ETF1 (NP_004721.1, 338 a.a. ~ 437 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 E~~N/A |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | ETF1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).
REFERENCES
Identification of a cellular factor that modulates HIV-1 programmed ribosomal frameshifting. Kobayashi Y, et al. J Biol Chem, 2010 Jun 25. PMID 20418372.Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.Does glutamine methylation affect the intrinsic conformation of the universally conserved GGQ motif in ribosomal release factors? And?r M, et al. Biochemistry, 2009 Apr 21. PMID 19265422.[Interface of the interaction of the middle domain of human translation termination factor eRF1 with eukaryotic ribosomes] Ivanova EV, et al. Mol Biol (Mosk), 2008 Nov-Dec. PMID 19140327.HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1. Figaro S, et al. FEBS Lett, 2008 Jul 9. PMID 18539146.
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