FBXW7 Antibody (monoclonal) (M02)

Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Three transcript variants encoding three different isoforms have been found for this gene.

REFERENCES

1.FBxW7 as a predictor of outcomes in ovarian cancer.Dickson EL, Vogel R, Leung S, Chow C, Huntsman D, Gilks B, Subramanian S.Journal of the American College of Surgeons Volume 217, Issue 3, Supplement, September 2013, Pages S72-732.Genomic and molecular characterization of esophageal squamous cell carcinoma.Lin DC, Hao JJ, Nagata Y, Xu L, Shang L, Meng X, Sato Y, Okuno Y, Varela AM, Ding LW, Garg M, Liu LZ, Yang H, Yin D, Shi ZZ, Jiang YY, Gu WY, Gong T, Zhang Y, Xu X, Kalid O, Shacham S, Ogawa S, Wang MR, Koeffler HPNat Genet. 2014 May;46(5):467-73. doi: 10.1038/ng.2935. Epub 2014 Mar 30.3.FBXW7 Mediates Chemotherapeutic Sensitivity and Prognosis in NSCLC.Yokobori T, Yokoyama Y, Mogi A, Endoh H, Altan B, Kosaka T, Yamaki E, Yajima T, Tomizawa K, Azuma Y, Onozato R, Miyazaki T, Tanaka S, Kuwano HMol Cancer Res. 2013 Oct 28.4.Structural Basis for a Reciprocal Regulation between SCF and CSN.Enchev RI, Scott DC, da Fonseca PC, Schreiber A, Monda JK, Schulman BA, Peter M, Morris EP.Cell Rep. 2012 Sep 5.