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FKSG14 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant CENPK.

     
  • 1 - FKSG14 Antibody (monoclonal) (M01) AT2059a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (55.33 KDa) .
  • 1 - FKSG14 Antibody (monoclonal) (M01) AT2059a
    CENPK monoclonal antibody (M01), clone 1G2-1B10 Western Blot analysis of CENPK expression in HepG2 ( (Cat # AT2059a )
  • 3 - FKSG14 Antibody (monoclonal) (M01) AT2059a
    Immunofluorescence of monoclonal antibody to CENPK on HepG2 cell. [antibody concentration 10 ug/ml]
  • 10 - FKSG14 Antibody (monoclonal) (M01) AT2059a
    Detection limit for recombinant GST tagged FKSG14 is approximately 0.03ng/ml as a capture antibody.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, E
Primary Accession Q9BS16
Other Accession BC005400
Reactivity Human
Host mouse
Clonality monoclonal
Isotype IgG1 Kappa
Clone Names 1G2-1B10
Calculated MW 31655 Da
Additional info
Gene ID 64105
Other Names Centromere protein K, CENP-K, Interphase centromere complex protein 37, Protein AF-5alpha, p33, CENPK, ICEN37
Target/Specificity CENPK (AAH05400.1, 1 a.a. ~ 269 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsFKSG14 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas

BACKGROUND

CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).

REFERENCES

KNL1 and the CENP-H/I/K complex coordinately direct kinetochore assembly in vertebrates. Cheeseman IM, et al. Mol Biol Cell, 2008 Feb. PMID 18045986.Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells. Izuta H, et al. Genes Cells, 2006 Jun. PMID 16716197.The CENP-H-I complex is required for the efficient incorporation of newly synthesized CENP-A into centromeres. Okada M, et al. Nat Cell Biol, 2006 May. PMID 16622420.The human CENP-A centromeric nucleosome-associated complex. Foltz DR, et al. Nat Cell Biol, 2006 May. PMID 16622419.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.

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