癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
GAPDH Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant GAPDH.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | P04406 |
| Other Accession | NM_002046 |
| Reactivity | Human, Mouse, Rat |
| Host | Mouse |
| Clonality | monoclonal |
| Isotype | IgG1 Kappa |
| Clone Names | 3C2 |
| Calculated MW | 36053 Da |
| Gene ID | 2597 |
|---|---|
| Other Names | Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-, GAPDH, GAPD |
| Target/Specificity | GAPDH (NP_002037, 226 a.a. ~ 335 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 E~~N/A |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | GAPDH Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
REFERENCES
1.Receptor for Activated Protein Kinase C: Requirement for Efficient MicroRNA Function and Reduced Expression in Hepatocellular Carcinoma.Otsuka M, Takata A, Yoshikawa T, Kojima K, Kishikawa T, Shibata C, Takekawa M, Yoshida H, Omata M, Koike K.PLoS One. 2011;6(9):e24359. Epub 2011 Sep 15.2.Expression and roles of Slit/Robo in human ovarian cancer.Dai CF, Jiang YZ, Li Y, Wang K, Liu PS, Patankar MS, Zheng J.Histochem Cell Biol. 2011 Apr 5. [Epub ahead of print]3.Anti-angiogenic effect of triterpenoidal saponins from Polygala senega.Arai M, Hayashi A, Sobou M, Ishida S, Kawachi T, Kotoku N, Kobayashi M.J Nat Med. 2010 Nov 2. [Epub ahead of print]4.The Effect of Allelic Variation in Aldo-Keto Reductase 1C2 on the In Vitro Metabolism of Dihydrotestosterone.Takahashi RH, Grigliatti TA, Reid RE, Riggs KW.J Pharmacol Exp Ther. 2009 Jun;329(3):1032-9. Epub 2009 Mar 3.
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