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>   首页   >   产品   >   一抗   >   代谢   >   GCLM Antibody (monoclonal) (M01)   

GCLM Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant GCLM.

     
  • 1 - GCLM Antibody (monoclonal) (M01) AT2177a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (55.88 KDa) .
  • 1 - GCLM Antibody (monoclonal) (M01) AT2177a
    GCLM monoclonal antibody (M01), clone 2B8 Western Blot analysis of GCLM expression in HeLa ( (Cat # AT2177a )
  • 10 - GCLM Antibody (monoclonal) (M01) AT2177a
    Detection limit for recombinant GST tagged GCLM is approximately 1ng/ml as a capture antibody.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P48507
Other Accession BC041809
Reactivity Human
Host mouse
Clonality monoclonal
Isotype IgG2a Kappa
Clone Names 2B8
Calculated MW 30727 Da
Additional info
Gene ID 2730
Other Names Glutamate--cysteine ligase regulatory subunit, GCS light chain, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate--cysteine ligase modifier subunit, GCLM, GLCLR
Target/Specificity GCLM (AAH41809, 1 a.a. ~ 274 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsGCLM Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas

BACKGROUND

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

REFERENCES

1.Protection against 2-chloroethyl ethyl sulfide (CEES) - Induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway.Abel EL, Bubel JD, Simper MS, Powell L, McClellan SA, Andreeff M, Macleod MC, Digiovanni J.Toxicol Appl Pharmacol. 2011 Jun 23. [Epub ahead of print]2.Molecular and Functional Characterization of Gastrula Organizer Cells Derived from Human Embryonic Stem Cells.Sharon N, Mor I, Golan-Lev T, Fainsod A, Benvenisty N.Stem Cells. 2011 Feb 15. doi: 10.1002/stem.621. [Epub ahead of print]3.Diversity in Antioxidant Response Enzymes in Progressive Stages of Human Nonalcoholic Fatty Liver Disease.Hardwick RN, Fisher CD, Canet MJ, Lake AD, Cherrington NJ.Drug Metab Dispos. 2010 Dec;38(12):2293-301. Epub 2010 Aug 30.4.Induction of Antioxidant Enzymes by Curcumin and Its Analogues in Human Islets: Implications in Transplantation.Balamurugan AN, Akhov L, Selvaraj G, Pugazhenthi S.Pancreas. 2009 May;38(4):454-60.

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