癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
GFI1 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant GFI1.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC, IF, E |
|---|---|
| Primary Accession | Q99684 |
| Other Accession | NM_005263 |
| Reactivity | Human |
| Host | mouse |
| Clonality | monoclonal |
| Isotype | IgG1 Kappa |
| Clone Names | 3G8 |
| Calculated MW | 45297 Da |
| Gene ID | 2672 |
|---|---|
| Other Names | Zinc finger protein Gfi-1, Growth factor independent protein 1, Zinc finger protein 163, GFI1, ZNF163 |
| Target/Specificity | GFI1 (NP_005254, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IHC~~1:100~500 IF~~1:50~200 E~~N/A |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | GFI1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene.
REFERENCES
Tag-SNP analysis of the GFI1-EVI5-RPL5-FAM69 risk locus for multiple sclerosis. Alcina A, et al. Eur J Hum Genet, 2010 Jul. PMID 20087403.A variant allele of Growth Factor Independence 1 (GFI1) is associated with acute myeloid leukemia. Khandanpour C, et al. Blood, 2010 Mar 25. PMID 20075157.The growth factor independence-1 (Gfi1) is overexpressed in chronic myelogenous leukemia. Huang M, et al. Acta Haematol, 2010. PMID 19887785.Contributions to neutropenia from PFAAP5 (N4BP2L2), a novel protein mediating transcriptional repressor cooperation between Gfi1 and neutrophil elastase. Salipante SJ, et al. Mol Cell Biol, 2009 Aug. PMID 19506020.Gfi-1 represses CDKN2B encoding p15INK4B through interaction with Miz-1. Basu S, et al. Proc Natl Acad Sci U S A, 2009 Feb 3. PMID 19164764.
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