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MED6 Antibody (monoclonal) (M07)

Mouse monoclonal antibody raised against a full-length recombinant MED6.

     
  • 1 - MED6 Antibody (monoclonal) (M07) AT2832a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (52.8 KDa) .
  • 1 - MED6 Antibody (monoclonal) (M07) AT2832a
    Western Blot analysis of MED6 expression in transfected 293T cell line by MED6 monoclonal antibody (M07), clone 4C2.

    Lane 1: MED6 transfected lysate(28.4 KDa).
    Lane 2: Non-transfected lysate.
  • 2 - MED6 Antibody (monoclonal) (M07) AT2832a
    Immunoperoxidase of monoclonal antibody to MED6 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession O75586
Other Accession BC004106
Reactivity Human
Host mouse
Clonality monoclonal
Isotype IgG2b Kappa
Clone Names 4C2
Calculated MW 28425 Da
Additional Information
Gene ID 10001
Other Names Mediator of RNA polymerase II transcription subunit 6, Activator-recruited cofactor 33 kDa component, ARC33, Mediator complex subunit 6, hMed6, Renal carcinoma antigen NY-REN-28, MED6, ARC33
Target/Specificity MED6 (AAH04106, 1 a.a. ~ 246 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
IHC~~1:100~500
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsMED6 Antibody (monoclonal) (M07) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription. Zhang X, et al. Mol Cell, 2005 Jul 1. PMID 15989967.PARP-1 determines specificity in a retinoid signaling pathway via direct modulation of mediator. Pavri R, et al. Mol Cell, 2005 Apr 1. PMID 15808511.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology. Sato S, et al. Mol Cell, 2004 Jun 4. PMID 15175163.The activator-recruited cofactor/Mediator coactivator subunit ARC92 is a functionally important target of the VP16 transcriptional activator. Yang F, et al. Proc Natl Acad Sci U S A, 2004 Feb 24. PMID 14983011.

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