NEK10 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant NEK10.

Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.64 KDa) .
NEK10 monoclonal antibody (M01), clone 1C9 Western Blot analysis of NEK10 expression in HeLa ( (Cat # AT3013a )
NEK10 monoclonal antibody (M01), clone 1C9. Western Blot analysis of NEK10 expression in Raw 264.7 ( (Cat # AT3013a )
NEK10 monoclonal antibody (M01), clone 1C9. Western Blot analysis of NEK10 expression in NIH/3T3 ( (Cat # AT3013a )
Western Blot analysis of NEK10 expression in transfected 293T cell line by NEK10 monoclonal antibody (M01), clone 1C9.

Lane 1: NEK10 transfected lysate(53.1 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence of monoclonal antibody to NEK10 on HeLa cell. [antibody concentration 10 ug/ml]
Detection limit for recombinant GST tagged NEK10 is approximately 0.03ng/ml as a capture antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, E
Primary Accession	Q6ZWH5
Other Accession	NM_152534
Reactivity	Human, Mouse
Host	mouse
Clonality	monoclonal
Isotype	IgG2a Kappa
Clone Names	1C9
Calculated MW	133259 Da

Additional Information
Gene ID	152110
Other Names	Serine/threonine-protein kinase Nek10, Never in mitosis A-related kinase 10, NimA-related protein kinase 10, NEK10
Target/Specificity	NEK10 (NP_689747, 211 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution	WB~~1:500~1000 IF~~1:50~200 E~~N/A
Format	Clear, colorless solution in phosphate buffered saline, pH 7.2 .
Storage	Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Precautions	NEK10 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.