癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
RAD18 Antibody (monoclonal) (M03)
Mouse monoclonal antibody raised against a partial recombinant RAD18.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF |
|---|---|
| Primary Accession | Q9NS91 |
| Other Accession | NM_020165 |
| Reactivity | Human, Rat |
| Host | mouse |
| Clonality | monoclonal |
| Isotype | IgG2b Kappa |
| Clone Names | 2B9 |
| Calculated MW | 56223 Da |
| Gene ID | 56852 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase RAD18, 632-, Postreplication repair protein RAD18, hHR18, hRAD18, RING finger protein 73, RAD18, RNF73 |
| Target/Specificity | RAD18 (NP_064550, 332 a.a. ~ 430 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IF~~1:50~200 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | RAD18 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
REFERENCES
A Large-scale genetic association study of esophageal adenocarcinoma risk. Liu CY, et al. Carcinogenesis, 2010 Jul. PMID 20453000.RAD18-dependent recruitment of SNM1A to DNA repair complexes by a ubiquitin-binding zinc finger. Yang K, et al. J Biol Chem, 2010 Jun 18. PMID 20385554.Multiple genetic variants in telomere pathway genes and breast cancer risk. Shen J, et al. Cancer Epidemiol Biomarkers Prev, 2010 Jan. PMID 20056641.Mutation analysis of Rad18 in human cancer cell lines and non small cell lung cancer tissues. Nakamura T, et al. J Exp Clin Cancer Res, 2009 Jul 25. PMID 19630985.Physical and functional interaction between WRNIP1 and RAD18. Yoshimura A, et al. Genes Genet Syst, 2009 Apr. PMID 19556710.
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