RPS27A Antibody (monoclonal) (M01)

Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

REFERENCES

1.Altered dynamics of ubiquitin hybrid proteins during tumor cell apoptosis.Han XJ, Lee MJ, Yu GR, Lee ZW, Bae JY, Bae YC, Kang SH, Kim DG.Cell Death Dis. 2012 Jan 19;3:e255. doi: 10.1038/cddis.2011.142.2.The HBx protein of hepatitis B virus regulates the expression, intracellular distribution and functions of Ribosomal Protein S27a.Fatima G, Mathan G, Kumar V.J Gen Virol. 2011 Dec 7.3.Chemoprevention with Aqueous Extract of Butea monosperma flowers results in normalization of nuclear morphometry and inhibition of a proliferation marker in liver tumors.Mathan G, Fatima G, Saxena AK, Chandan BK, Jaggi BS, Gupta BD, Qazi GN, Balasundaram C, Anand Rajan KD, Kumar VL, Kumar V.Phytother Res. 2010 Jul 28. [Epub ahead of print]