癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
USP15 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a full length recombinant USP15.
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IF |
|---|---|
| Primary Accession | Q9Y4E8 |
| Other Accession | BC020688 |
| Reactivity | Human |
| Host | mouse |
| Clonality | monoclonal |
| Isotype | IgG1 kappa |
| Clone Names | 1C10 |
| Calculated MW | 112419 Da |
| Gene ID | 9958 |
|---|---|
| Other Names | Ubiquitin carboxyl-terminal hydrolase 15, Deubiquitinating enzyme 15, Ubiquitin thioesterase 15, Ubiquitin-specific-processing protease 15, Unph-2, Unph4, USP15, KIAA0529 |
| Target/Specificity | USP15 (AAH20688, 1 a.a. ~ 235 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IF~~1:50~200 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | USP15 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Ubiquitin (MIM 191339), a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). Also see USP1 (MIM 603478).
REFERENCES
1.The Ubiquitin-Specific Protease USP15 Promotes RIG-I–Mediated Antiviral Signaling by Deubiquitylating TRIM25.Pauli EK, Chan YK, Davis ME, Gableske S, Wang MK, Feister KF, Gack MU.Science Signaling 7 (307), ra3.2.The Ubiquitin-Specific Protease USP15 Promotes RIG-I-Mediated Antiviral Signaling by Deubiquitylating TRIM25.Pauli EK, Chan YK, Davis ME, Gableske S, Wang MK, Feister KF, Gack MUSci Signal. 2014 Jan 7;7(307):ra3. doi: 10.1126/scisignal.2004577.3.The Human COP9 Signalosome Protects Ubiquitin-conjugating Enzyme 3 (UBC3/Cdc34) from {beta}-Transducin Repeat-containing Protein ({beta}TrCP)-mediated Degradation.Fernandez-Sanchez ME, Sechet E, Margottin-Goguet F, Rogge L, Bianchi E.J Biol Chem. 2010 Jun 4;285(23):17390-7. Epub 2010 Apr 8.4.COP9 Signalosome Interacts ATP-dependently with p97/Valosin-containing Protein (VCP) and Controls the Ubiquitination Status of Proteins Bound to p97/VCP.Cayli S, Klug J, Chapiro J, Frohlich S, Krasteva G, Orel L, Meinhardt A.J Biol Chem. 2009 Dec 11;284(50):34944-53. Epub 2009 Oct 13.5.USP15 plays an essential role for caspase-3 activation during Paclitaxel-induced apoptosis.Xu M, Takanashi M, Oikawa K, Tanaka M, Nishi H, Isaka K, Kudo M, Kuroda M.Biochem Biophys Res Commun. 2009 Oct 16;388(2):366-71. Epub 2009 Aug 8.6.The Ubiquitin Specific Peptidase USP15 Regulates Human Papilloma Virus 16 E6 Protein Stability.Vos RM, Altreuter J, White EA, Howley PM.J Virol. 2009 Sep;83(17):8885-92. Epub 2009 Jun 24.7.The COP9/signalosome increases the efficiency of pVHL ubiquitin ligase-mediated hypoxia inducible factor-alpha ubiquitination.Miyauchi Y, Kato M, Tokunaga F, Iwai K.J Biol Chem. 2008 Jun 13;283(24):16622-16631. Epub 2008 Apr 18.8.CSN controls NF-kappaB by deubiquitinylation of IkappaBalpha.Schweitzer K, Bozko PM, Dubiel W, Naumann M.EMBO J. 2007 Mar 21;26(6):1532-41. Epub 2007 Feb 22.
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