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PAH Antibody (Center)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - PAH Antibody (Center) AW5074
    Western blot analysis of lysates from mouse liver,rat liver tissue,HepG2 cell line (from left to right), using PAH Antibody (Center)(Cat. #AW5074). AW5074 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
  • 14 - PAH Antibody (Center) AW5074
    Immunohistochemical analysis of paraffin-embedded H. liver section using PAH Antibody (Center)(Cat#AW5074). AW5074 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - PAH Antibody (Center) AW5074
    Immunohistochemical analysis of paraffin-embedded H. kidney section using PAH Antibody (Center)(Cat#AW5074). AW5074 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - PAH Antibody (Center) AW5074
    Immunohistochemical analysis of paraffin-embedded M. kidney section using PAH Antibody (Center)(Cat#AW5074). AW5074 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB
Primary Accession P00439
Other Accession P04176, P16331, Q2KIH7
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Mouse
Clonality Monoclonal
Calculated MW 51862 Da
Isotype IgG1,κ
Antigen Source HUMAN
Additional Information
Gene ID 5053
Antigen Region 127-161 aa
Other Names Phenylalanine-4-hydroxylase, PAH, Phe-4-monooxygenase, PAH
Dilution IHC-P~~1:100~500
WB~~1:1000
Target/Specificity This PAH antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 127-161 amino acids from the Central region of human PAH.
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPAH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PAH
Function Catalyzes the hydroxylation of L-phenylalanine to L-tyrosine.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

REFERENCES

Kwok S.C.M.,et al.Biochemistry 24:556-561(1985).
Scriver C.R.,et al.Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
Cotton R.G.,et al.Biochem. J. 255:193-196(1988).
Miranda F.F.,et al.J. Biol. Chem. 277:40937-40943(2002).
Siltberg-Liberles J.,et al.Gene 427:86-92(2008).

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