DMRTA2 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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Western blot analysis of lysates from K562 cell line,zebra fish brain,mouse brain,mouse testis tissue lysate,PC-3 cell line (from left to right), using DMRTA2 Antibody (C-term)(Cat. #AW5104). AW5104 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
Fluorescent confocal image of U251 cell stained with DMRTA2 Antibody (C-term)(Cat#AW5104).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with DMRTA2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). DMRTA2 immunoreactivity is localized to Microtubules significantly.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IF, WB
Primary Accession	Q96SC8
Other Accession	A2A9A2, A6QQ94, NP_115486.1
Reactivity	Mouse, Human, Zebrafish
Predicted	Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	53356 Da
Isotype	Rabbit IgG
Antigen Source	HUMAN

Additional Information
Gene ID	63950
Antigen Region	487-515 aa
Other Names	DMRTA2; DMRT5; Doublesex- and mab-3-related transcription factor A2; Doublesex- and mab-3-related transcription factor 5
Dilution	IF~~1:10~50 WB~~1:1000
Target/Specificity	This DMRTA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-515 amino acids from the C-terminal region of human DMRTA2.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	DMRTA2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	DMRTA2
Synonyms	DMRT5
Function	May be involved in sexual development.
Cellular Location	Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00070}.
Tissue Location	Expressed in testis..

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.