|Application ||WB, IHC|
|Other Accession||P04961, P61258, P57761, Q3ZBW4|
|Calculated MW||H=29;M=29;Rat=29 KDa|
|Other Names||PCNA;Proliferating cell nuclear antigen; Proliferating cell nuclear antigen; Cyclin|
|Target/Specificity||This PCNA antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 236-261 amino acids from the C-terminal region of human PCNA.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PCNA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (PubMed:24695737).|
|Cellular Location||Nucleus. Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.|
Provided below are standard protocols that you may find useful for product applications.
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Almendral J.M., et al. Proc. Natl. Acad. Sci. U.S.A. 84:1575-1579(1987).
Travali S., et al. J. Biol. Chem. 264:7466-7472(1989).
Ota T., et al. Nat. Genet. 36:40-45(2004).
Deloukas P., et al. Nature 414:865-871(2001).
Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.