癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
GSS Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- 实验流程
- 背景知识
Application 
| IF, IHC-P, WB |
|---|---|
| Primary Accession | P48637 |
| Other Accession | P46413, P51855, Q8HXX5, Q5EAC2 |
| Reactivity | Human |
| Predicted | Mouse, Rat, Monkey, Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 52385 Da |
| Isotype | Rabbit IgG |
| Antigen Source | HUMAN |
| Gene ID | 2937 |
|---|---|
| Antigen Region | 372-400 aa |
| Other Names | GSS; Glutathione synthetase; Glutathione synthase |
| Dilution | IF~~1:25 IHC-P~~1:100~500 WB~~1:1000 |
| Target/Specificity | This GSS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 372-400 amino acids from the C-terminal region of human GSS. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GSS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GSS (HGNC:4624) |
|---|---|
| Function | Catalyzes the production of glutathione from gamma- glutamylcysteine and glycine in an ATP-dependent manner (PubMed:7646467, PubMed:9215686). Glutathione (gamma- glutamylcysteinylglycine, GSH) is the most abundant intracellular thiol in living aerobic cells and is required for numerous processes including the protection of cells against oxidative damage, amino acid transport, the detoxification of foreign compounds, the maintenance of protein sulfhydryl groups in a reduced state and acts as a cofactor for a number of enzymes (PubMed:10369661). Participates in ophthalmate biosynthesis in hepatocytes (By similarity). |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. GSS functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione.
REFERENCES
Starr,J.M., et.al., Mech. Ageing Dev. 129 (12), 745-751 (2008)
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Anonymous
2016-08-24 09:20:44
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Species tested
Human,Mouse,Rat
Application tested
WB
Organization tested
Hela,U87,A549,LO2,293T,HT1080,SW480,Kindey(M),Kindey(R)
Barcode encoding
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature.
2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃
3. Wash 5*5 min with TBST.
4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature.
5. Wash 5*5 min with TBST.
6. Incubate with ECL substrates and expose
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