|Application ||IF, IHC-P, WB|
|Other Accession||P46413, P51855, Q8HXX5, Q5EAC2|
|Predicted||Mouse, Rat, Monkey, Bovine|
|Calculated MW||H=52;M=52;Rat=52 KDa|
|Other Names||GSS; Glutathione synthetase; Glutathione synthase|
|Target/Specificity||This GSS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 372-400 amino acids from the C-terminal region of human GSS.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GSS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
Provided below are standard protocols that you may find useful for product applications.
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. GSS functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione.
Starr,J.M., et.al., Mech. Ageing Dev. 129 (12), 745-751 (2008)
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1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose