HMGCS1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

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Western blot analysis of lysates from mouse liver,rat liver,human liver tissue (from left to right), using HMGCS1 Antibody (Center)(Cat. #AW5269). AW5269 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of hela cells using HMGCS1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, IHC-P, WB
Primary Accession	Q01581
Other Accession	P17425, Q8JZK9, P13704, P23228
Reactivity	Mouse, Rat, Human
Predicted	Hamster, Chicken
Host	Rabbit
Clonality	Polyclonal
Calculated MW	57294 Da
Isotype	Rabbit IgG
Antigen Source	HUMAN

Additional Information
Gene ID	3157
Antigen Region	290-317 aa
Other Names	HMGCS1; HMGCS; Hydroxymethylglutaryl-CoA synthase, cytoplasmic; 3-hydroxy-3-methylglutaryl coenzyme A synthase
Dilution	FC~~1:10~50 IHC-P~~1:100~500 WB~~ 1:1000
Target/Specificity	This HMGCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-317 amino acids from the Central region of human HMGCS1.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	HMGCS1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	HMGCS1 (HGNC:5007)
Synonyms	HMGCS
Function	Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is converted by HMG-CoA reductase (HMGCR) into mevalonate, a precursor for cholesterol synthesis.
Cellular Location	Cytoplasm.
Tissue Location	Highly expressed in the cerebral cortex.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.