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NRAS Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - NRAS Antibody (C-term) AW5296
    Western blot analysis of lysates from MCF-7,A375 cell line (from left to right), using NRAS Antibody (C-term)(Cat. #AW5296). AW5296 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 14 - NRAS Antibody (C-term) AW5296
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with NRAS antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - NRAS Antibody (C-term) AW5296
    NRAS Antibody (C-term) (Cat. #AW5296) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB
Primary Accession P01111
Other Accession Q2MJK3
Reactivity Mouse, Rat, Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 21229 Da
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 4893
Antigen Region 147-179 aa
Other Names NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras
Dilution FC~~1:10~50
IHC-P~~1:100~500
WB~~1:1000
Target/Specificity This NRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-179 amino acids from the C-terminal region of human NRAS.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNRAS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NRAS
Synonyms HRAS1
Function Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor Note=Shuttles between the plasma membrane and the Golgi apparatus
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).

REFERENCES

Smalley,K.S., Cancer Res. 68 (14), 5743-5752 (2008)
Banerji,U., Mol. Cancer Ther. 7 (4), 737-739 (2008)

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