|Application ||IHC-P, FC, WB|
|Other Accession||O88498, O54918|
|Calculated MW||H=22,16,13,19,12,9,10,14,23;M=22,16,13;Rat=22,16,13,11 KDa|
|Other Names||BCL2L11; BIM; Bcl-2-like protein 11; Bcl2-interacting mediator of cell death|
|Target/Specificity||This BCL2L11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-160 amino acids from the Central region of human BCL2L11.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BCL2L11 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.|
|Cellular Location||Endomembrane system; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes. Isoform BimL: Mitochondrion. Isoform Bim-alpha1: Mitochondrion.|
|Tissue Location||Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are widely expressed with tissue- specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney|
Provided below are standard protocols that you may find useful for product applications.
BCL2L11 belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator.
Hippe,D.,et.al., J. Cell. Sci. 122 (PT 19), 3511-3521 (2009)
Putcha,G.V., et.al., Neuron 38 (6), 899-914 (2003)