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>   首页   >   产品   >   一抗   >   癌症   >   STRADA Antibody (C-term)   

STRADA Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - STRADA Antibody (C-term) AW5374
    All lanes : Anti-STRADA Antibody (C-term) at 1:1000 dilution Lane 1: 293T whole cell lysates Lane 2: human brain lysates Lane 3: mouse brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - STRADA Antibody (C-term) AW5374
    Flow cytometric analysis of SH-SY5Y cells using STRADA Antibody (C-term)(green, Cat#AW5374) compared to an isotype control of rabbit IgG(blue). AW5374 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 3 - STRADA Antibody (C-term) AW5374
    Fluorescent image of A549 cells stained with STRADA Antibody (C-term)(Cat#AW5374). AW5374 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - STRADA Antibody (C-term) AW5374
    Immunohistochemical analysis of paraffin-embedded H. duodenum section using STRADA Antibody (C-term)(Cat#AW5374). AW5374 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - STRADA Antibody (C-term) AW5374
    Immunohistochemical analysis of paraffin-embedded H. liver section using STRADA Antibody (C-term)(Cat#AW5374). AW5374 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - STRADA Antibody (C-term) AW5374
    Immunohistochemical analysis of paraffin-embedded M. duodenum section using STRADA Antibody (C-term)(Cat#AW5374). AW5374 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - STRADA Antibody (C-term) AW5374
    Immunohistochemical analysis of paraffin-embedded M. liver section using STRADA Antibody (C-term)(Cat#AW5374). AW5374 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, FC, WB
Primary Accession Q7RTN6
Other Accession Q3UUJ4, Q4R6X5
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 48369 Da
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 92335
Antigen Region 303-346 aa
Other Names STE20-related kinase adapter protein alpha, STRAD alpha, STE20-related adapter protein, Serologically defined breast cancer antigen NY-BR-96, STRADA, LYK5 {ECO:0000312|EMBL:AAP422801}, STRAD
Dilution IHC-P~~1:100~500
IF~~1:25
FC~~1:25
WB~~1:1000
Target/Specificity This STRADA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 303-346 amino acids from the C-terminal region of human STRADA.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTRADA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STRADA
Synonyms LYK5 {ECO:0000312|EMBL:AAP42280.1}, STRA
Function Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
Cellular Location Nucleus. Cytoplasm
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.

REFERENCES

Scanlan M.J.,et al.Cancer Immun. 1:4-4(2001).
Shan Y.X.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Zody M.C.,et al.Nature 440:1045-1049(2006).

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