|Application ||IF, FC, IHC-P, WB|
|Calculated MW||H=20,15;M=20;R=20 KDa|
|Other Names||Adenine phosphoribosyltransferase, APRT, APRT|
|Target/Specificity||This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-60 amino acids from the N-terminal region of human APRT.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||APRT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.|
Provided below are standard protocols that you may find useful for product applications.
A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.
Silva,C.H., et. al.,J. Biomol. Struct. Dyn. 25 (6), 589-597 (2008)
Di Pietro,V., et. al., Clin. Biochem. 40 (1-2), 73-80 (2007)