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APRT Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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All lanes : Anti-APRT Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: A2058 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with APRT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of HepG2 cells using APRT Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Fluorescent image of A549 cell stained with APRT Antibody (N-term)(Cat#AW5489/SA091009AF).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with APRT primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).APRT immunoreactivity is localized to Cytoplasm and Nucleus significantly.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IF, FC, IHC-P, WB
Primary Accession	P07741
Reactivity	Mouse, Rat, Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	19608 Da
Isotype	Rabbit IgG
Antigen Source	HUMAN

Additional Information
Gene ID	353
Antigen Region	27-60 aa
Other Names	Adenine phosphoribosyltransferase, APRT, APRT
Dilution	IF~~1:10~50 FC~~1:10~50 IHC-P~~1:100~500 WB~~1:1000
Target/Specificity	This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-60 amino acids from the N-terminal region of human APRT.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	APRT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	APRT (HGNC:626)
Function	Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
Cellular Location	Cytoplasm.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.

REFERENCES

Silva,C.H., et. al.,J. Biomol. Struct. Dyn. 25 (6), 589-597 (2008)
Di Pietro,V., et. al., Clin. Biochem. 40 (1-2), 73-80 (2007)

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¥ 2,600.00

Cat# AW5489

Size:

100 µl

Price:

¥ 2,600.00

Quantity:

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Availability: In Stock

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APRT Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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