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(Mouse) Epcam Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

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AW5496 staining Epcam in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
AW5496 staining Epcam in Human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Overlay histogram showing HepG2 cells stained with AW5496 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AW5496, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-Epcam Antibody (C-term) at 1:1000 dilution Lane 1: mouse kidney lysates Lane 2: mouse lung lysates Lane 3: mouse testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IHC-P
Primary Accession	Q99JW5
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	35019 Da
Isotype	Rabbit IgG
Antigen Source	HUMAN

Additional Information
Gene ID	17075
Antigen Region	302-335 aa
Other Names	Epithelial cell adhesion molecule, Ep-CAM, Epithelial glycoprotein 314, EGP314, mEGP314, Protein 289A, Tumor-associated calcium signal transducer 1, CD326, Epcam, Tacstd1
Dilution	WB~~1:1000 FC~~1:25 IHC-P~~1:100~500
Target/Specificity	This mouse Epcam antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 302-335 amino acids from the C-terminal region of mouse Epcam.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	(Mouse) Epcam Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	Epcam
Synonyms	Tacstd1
Function	May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).
Cellular Location	Lateral cell membrane {ECO:0000250\|UniProtKB:P16422}; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P16422}. Cell junction, tight junction {ECO:0000250\|UniProtKB:P16422}. Note=Colocalizes with CLDN7 at the lateral cell membrane and tight junction {ECO:0000250\|UniProtKB:P16422}

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E (By similarity).

REFERENCES

Bergsagel P.L.,et al.J. Immunol. 148:590-596(1992).
Carninci P.,et al.Science 309:1559-1563(2005).

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¥ 2,600.00

Cat# AW5496

Size:

100 µl

Price:

¥ 2,600.00

Quantity:

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Availability: In Stock

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(Mouse) Epcam Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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