|Application ||IHC-P, WB|
|Calculated MW||H=108,109 KDa|
|Other Names||General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, TAP, Vesicle-docking protein, USO1, VDP|
|Target/Specificity||This USO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 932-960 amino acids from the C-terminal region of human USO1.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||USO1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.|
|Cellular Location||Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein Note=Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.|
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner.
Striegl, H., et al. PLoS ONE 5 (2), E8991 (2010) :
Merk, M., et al. J. Immunol. 182(11):6896-6906(2009)
Mukherjee, S., et al. J. Biol. Chem. 284(3):1709-1717(2009)
Striegl, H., et al. PLoS ONE 4 (2), E4656 (2009) :
Guo, Y., et al. Mol. Biol. Cell 19(7):2830-2843(2008)