|Application ||IHC-P, WB|
|Calculated MW||H=31;M=32 KDa|
|Other Names||U1 small nuclear ribonucleoprotein A, U1 snRNP A, U1-A, U1A, SNRPA|
|Target/Specificity||This SNRPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-111 amino acids from the Central region of human SNRPA.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SNRPA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.|
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene associates with stem loop II of the U1 small nuclear ribonucleoprotein, which binds the 5' splice site of precursor mRNAs and is required for splicing. The encoded protein autoregulates itself by polyadenylation inhibition of its own pre-mRNA via dimerization and has been implicated in the coupling of splicing and polyadenylation.
Guan, F., et al. RNA 13(12):2129-2140(2007)
Hall-Pogar, T., et al. RNA 13(7):1103-1115(2007)
Shajani, Z., et al. J. Mol. Biol. 349(4):699-715(2005)
Andersen, J.S., et al. Nature 433(7021):77-83(2005)
Jessen, T.H., et al. EMBO J. 10(11):3447-3456(1991)