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DANRE foxh1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - DANRE foxh1 Antibody (Center) Azb10035a
    All lanes : Anti-(DANRE) foxh1 Antibody (Center) at 1:1000 dilution Lane 1: Zebrafish lysate Lane 2: ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - DANRE foxh1 Antibody (Center) Azb10035a
    DANRE foxh1 Antibody (Center) (Cat. #Azb10035a) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the DANRE foxh1 antibody detected the DANRE foxh1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9I9E1
Reactivity Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 51620 Da
Antigen Region 313-334 aa
Additional Information
Gene ID 57930
Other Names Forkhead box protein H1, Forkhead activin signal transducer 1, Fast-1, Schmalspur protein, foxh1, fast1, sur
Target/Specificity This DANRE foxh1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-334 amino acids from the Central region of DANRE foxh1.
Dilution WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDANRE foxh1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name foxh1
Synonyms fast1, sur
Function Transcriptional activator. Activates an activin response element (ARE). Recognizes and binds to the DNA sequence 5'- TGT[GT][GT]ATT-3'. Modulator of nodal signaling required for organizer formation. Also required for the development of dorsal axial structures and left-right symmetry.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00089, ECO:0000269|PubMed:10996071}
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Transcriptional activator. Activates an activin response element (ARE). Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Modulator of nodal signaling required for organizer formation. Also required for the development of dorsal axial structures and left-right symmetry.

REFERENCES

Pogoda H.-M., et al. Curr. Biol. 10:1041-1049(2000).
Sirotkin H.I., et al. Curr. Biol. 10:1051-1054(2000).
Boggetti B., et al. Mech. Dev. 99:187-190(2000).

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