NRG-1β2

Product Information
Primary Accession	Q02297-7
Species	Human
Sequence	Ser177-Gln237
Purity	> 96% as analyzed by SDS-PAGE > 96% as analyzed by HPLC
Endotoxin Level	< 1 EU/ µg of protein by LAL method
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 50.0 ng/ml, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg.
Expression System	E. coli
Theoretical Molecular Weight	7.0 kDa
Formulation	Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH 7.0, containing 0.5 % HAS and 2 % mannitol.
Reconstitution	It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
Storage & Stability	Upon receiving, this product remains stable for up to 6 months at -20°C or -70°C. Upon reconstitution, the product should be stable for up to 1 week at 2-8°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles.

Additional Information
Target Background	Neuregulin is a signaling protein for ErbB2/ErbB4 receptor heterodimers on the cardiac muscle cells, playing an important role in heart structure and function through inducing ErbB2/ErbB4 receptor phosphorylation and cardiomyocyte differentiation. Research on molecular level discovered that recombinant neuregulin could make disturbed myocardial cell structure into order and strengthen the connection between myocardial cells by intercalated discs re-organization.