癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
LIF
- 产品详情
- 实验流程
| Primary Accession | P09056 |
|---|---|
| Species | Mouse |
| Sequence | Ser24-Phe203 |
| Purity | > 95% as analyzed by SDS-PAGE > 95% as analyzed by HPLC |
| Endotoxin Level | < 0.2 EU/ µg of protein by gel clotting method |
| Biological Activity | ED50 < 0.01 ng/ml, measured by a cell differentiation assay using M1 cells, corresponding to a specific activity of > 1.0 × 108 units/mg. |
| Expression System | E. coli |
| Formulation | Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0. |
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 µg/ml. |
| Storage & Stability | Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Gene ID | 16878 |
|---|---|
| Other Names | Leukemia inhibitory factor, LIF, Differentiation-stimulating factor, D factor, Lif |
| Target Background | Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the long four-helix bundle cytokine superfamily. LIF shares tertiary structure with several other cytokines, including Interleukin-6 (IL-6), Oncostatin M, ciliary neurotropic factor, and cardiotrophin-1, and their functions in vivo are also redundant to some extent. LIF can bind to the common receptor of IL-6 subfamily, gp130, and then recruit its own receptor LIF Receptor to form a ternary complex. The basal expression of LIF in vivo is low; and its expression is induced by pro-inflammatory factors, including lipopolysaccharide, IL-1, and IL-17, and inhibited by anti-inflammatory agents, including IL-4 and IL-13. The functions of LIF include proliferation of primordial germ cells, regulation in blastocyst implantation and early pregnancy, and maintenance of pluripotent embryonic stem cells. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Lif {ECO:0000312|MGI:MGI:96787} |
|---|---|
| Function | Functions as a cytokine that binds to the LIF receptor complex (By similarity). The LIF receptor complex indeed consists of two signaling receptor subunits IL6ST/gp130 and LIFR that transduce the signal into the cell (By similarity). Functionally, regulates cell self-renewal, differentiation and survival of embryonic stem cells (EScs) and is crucial for embryonic implantation (PubMed:1522892, PubMed:16452727, PubMed:26775702, PubMed:3127201, PubMed:3143916). Mechanistically, ligand binding to LIFR, induces heterodimerization with IL6ST/gp130 activating JAK tyrosine kinases (JAK1 or JAK2 and to lesser extent TYK2) bound to their intracellular domains (PubMed:8921810). These kinases subsequently phosphorylate IL6ST/gp130 and LIFR (PubMed:8921810). The tyrosine phosphorylated signaling receptors serve in turn as docking sites for recruitment and activation of signal transducers and activators of transcription (STAT3 and to lesser extent STAT1) (PubMed:8921810). In parallel to the activation of the STAT3-pathway, the binding of LIF to the LIF receptor complex leads to the activation of the mitogen-activated protein kinase (MAPK) and the phosphatidylinositol 3-kinase (PI(3)K) pathways (PubMed:15328362, PubMed:9545305). Tyrosine phosphorylation of the p85 subunit of PI(3)K by JAK1 leads to activation of the serine/ threonine kinase AKT (protein kinase B) (PubMed:9545305). PI(3)K-dependent signaling is required for efficient self-renewal of murine ES cells, and regulation of ERK activity is functionally important in this response (PubMed:15328362). Moreover, induces tyrosine phosphorylation of PTPN11, that associates with IL6ST/gp130-LIFR, which in turn recruits the p85 regulatory subunit of PI(3)K protein kinase, promoting PI3K-AKT activation, and (in many systems) GRB2-RAS-MAPK signaling (By similarity). |
| Cellular Location | Secreted {ECO:0000250|UniProtKB:P15018}. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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