CD73

Product Information
Primary Accession	P21589
Species	Human
Sequence	Trp27-Lys547
Purity	> 95% as analyzed by SDS-PAGE > 95% as analyzed by HPLC
Endotoxin Level	≤ 1 EU/ µg of protein by LAL method
Biological Activity	Immobilized Human CD73, His at 0.5 µg/ml (100 µl/Well). Dose response curve for Anti-CD73 Ab with the EC₅₀ of 19.3 ng/ml determined by ELISA.
Expression System	Expi293
Formulation	Supplied as a 0.22 µm filtered solution in 20 mM Tris, 120 mM NaCl, 4 mM CaCl2, 20% glycerol, pH 7.5.
Storage & Stability	Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Avoid repeated freeze-thaw cycles.

Additional Information
Gene ID	4907
Other Names	5'-nucleotidase, 5'-NT, 3.1.3.35, 3.1.3.5, 3.1.3.89, 3.1.3.91, 3.1.3.99, 5'-deoxynucleotidase, Ecto-5'-nucleotidase, IMP-specific 5'-nucleotidase, Thymidylate 5'-phosphatase, CD73, NT5E, NT5, NTE
Target Background	CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A deficiency of CD73 occurs in a variety of immunodeficiency diseases.

Protein Information
Name	NT5E
Synonyms	NT5, NTE
Function	Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084).
Cellular Location	Cell membrane; Lipid-anchor, GPI-anchor