癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
OSM
- 产品详情
- 实验流程
| Primary Accession | Q65Z15 |
|---|---|
| Species | Rat |
| Sequence | Lys26-Arg239 |
| Purity | > 96% as analyzed by SDS-PAGE > 96% as analyzed by HPLC |
| Endotoxin Level | < 1 EU/ µg of protein by LAL method |
| Biological Activity | The ED50 as determined by a cell proliferation assay using murine NIH-3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of 5.0 × 105 IU/mg. |
| Expression System | E. coli |
| Theoretical Molecular Weight | 24.3 kDa |
| Formulation | Lyophilized from a 0.2 µm filtered solution in 2 × PBS, 0.1 % Tween-80. |
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. |
| Storage & Stability | Upon receiving, this product remains stable for up to 6 months at -20°C or -70°C. Upon reconstitution, the product should be stable for up to 1 week at 2-8°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
| Gene ID | 289747 |
|---|---|
| Other Names | Oncostatin-M, OSM, Osm |
| Target Background | Oncostatin-M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. Furthermore, the effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant murine OSM contains 215 amino acids and has a molecular mass of 22.4 kDa. It has approximately 48 % and 72 % amino acid sequence identity with human and murine OSM. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Osm {ECO:0000312|RGD:1585012} |
|---|---|
| Function | Functions as a cytokine that uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST) (By similarity). Functionally, regulates many processes including cell proliferation, cell differentiation, cytokine production (PubMed:15743783). Mechanistically, low affinity ligand binding to IL6ST/gp130, induces heterodimerization with LIFR or OSMR, activating JAK tyrosine kinases (JAK1 or JAK2 and to a lesser extent TYK2) bound to their intracellular domains. These kinases subsequently phosphorylate IL6ST/gp130 and LIFR or OSMR. The tyrosine phosphorylated signaling receptors serve in turn as docking sites for recruitment and activation of STAT3. The type II OSM complex receptor is also able in addition to STAT3 to recruit STAT5B. Moreover, the type II OSM complex receptor recruits SHC1 through OSMR in a JAK1 mediated phosphotyrosine-dependent manner, leading to SHC1 association with GRB2 and downstream activation of the Ras/Raf/MAPK pathway (By similarity). |
| Cellular Location | Secreted {ECO:0000250|UniProtKB:P13725}. |
| Tissue Location | Widely expressed. Expressed at higher levels in liver, skin and spleen. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。
如有疑问，联系：0512-88856768 tech-china@abcepta.com.
