BSA
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- 实验流程
| Primary Accession | P02769 |
|---|---|
| Species | Bovine |
| Sequence | Asp25-Ala607 |
| Purity | ≥ 95% as analyzed by SDS-PAGE |
| Endotoxin Level | < 0.2 EU/ µg of protein by gel clotting method |
| Expression System | P. pastoris |
| Theoretical Molecular Weight | 67.3 kDa |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4 |
| Reconstitution | Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O up to 100 µg/ml |
| Storage & Stability | Upon receiving, this product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable up to 1 week at 4 °C or up to 3 months at -20 °C. Avoid repeated freeze-thaw cycles. |
| Gene ID | 280717 |
|---|---|
| Other Names | Albumin, BSA, Bos d 6, ALB |
| Target Background | Bovine serum albumin (BSA) is a soluble monomeric protein. It is widely as a supplement in biochemical and tissue culture media, promoting cell growth and survival. BSA stabilizes extracellular fluid volume and functions as a carrier for small molecules such as steroids, fatty acids, and thyroid hormones. It is also used in drug development, protein purification, and food processing. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ALB |
|---|---|
| Function | Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (By similarity). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (Probable). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (PubMed:22677715). The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood (Probable). The rank order of affinity is zinc > calcium > magnesium (Probable). Binds to the bacterial siderophore enterobactin and inhibits enterobactin- mediated iron uptake of E.coli, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017). |
| Cellular Location | Secreted. |
| Tissue Location | Plasma. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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Cat# PVGS1758
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