CD3E&CD3D
- 产品详情
- 实验流程
Primary Accession | Q95LI5.2(CD3E)&Q95LI8(CD3D) |
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Species | Cynomolgus |
Sequence | Gln22-Asp117 (CD3E)&Phe22-Ala105 (CD3D) |
Purity | > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC |
Endotoxin Level | Less than 1EU per µg by the LAL method. |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized CD3E&CD3D, His & Flag, Cynomolgus at 0.5 µg/ml (100 µl/well) on the plate can bind Anti-CD3 Antibody, hFc Tag. Test result was comparable to standard batch. |
Expression System | HEK293 |
Theoretical Molecular Weight | 11.93 kDa (CD3E) and 10.55 kDa (CD3D) |
Formulation | Lyophilized from a 0.22 µm filtered solution in PBS,(pH 7.4). |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 µg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Target Background | T-cell surface glycoprotein CD3 epsilon&CD3 delta chain, also known as CD3E&CD3D, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. |
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Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.

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