癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-VEGF Antibody
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Application 
| WB |
|---|---|
| Primary Accession | Q00731 |
| Host | Rabbit |
| Reactivity | Mouse, Rat |
| Clonality | Polyclonal |
| Format | Lyophilized |
| Description | Rabbit IgG polyclonal antibody for Vascular endothelial growth factor A(VEGFA) detection. Tested with WB in Mouse;Rat. |
| Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Gene ID | 22339 |
|---|---|
| Other Names | Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, Vegfa, Vegf |
| Calculated MW | 43131 Da |
| Application Details | Western blot, 0.1-0.5 µg/ml, Rat, Mouse |
| Subcellular Localization | Isoform VEGF-1: Secreted. |
| Tissue Specificity | In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults. |
| Source | Eukaryota |
| Protein Name | Vascular endothelial growth factor A |
| Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Immunogen | E.coli-derived mouse VEGF recombinant protein (Position: A27-R214). Mouse VEGF shares 89.4% and 98.4% amino acid (aa) sequence identity with human and rat VEGF, respectively. |
| Purification | Immunogen affinity purified. |
| Cross Reactivity | No cross reactivity with other proteins. |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Vegfa |
|---|---|
| Synonyms | Vegf |
| Function | [N-VEGF]: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed:35455969). Involved in protecting cells from hypoxia- mediated cell death (PubMed:35455969). |
| Cellular Location | [N-VEGF]: Cytoplasm {ECO:0000250|UniProtKB:P15692}. Nucleus {ECO:0000250|UniProtKB:P15692} Note=Cytoplasmic in normoxic conditions and localizes to the nucleus under hypoxic conditions. {ECO:0000250|UniProtKB:P15692} [Isoform L-VEGF-2]: Endoplasmic reticulum {ECO:0000250|UniProtKB:P15692}. Golgi apparatus {ECO:0000250|UniProtKB:P15692}. Secreted, extracellular space, extracellular matrix {ECO:0000250|UniProtKB:P15692} [Isoform VEGF-2]: Secreted. |
| Tissue Location | In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression continues in kidney glomeruli and choroid plexus in adults |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. And VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. This gene can also regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.
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