Anti-Bim BCL2L11 Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:50 dilution.
Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Western blot analysis of Bim expression in A431 cell lysate.
Immunohistochemical analysis of paraffin-embedded human cervix cancer, using Bim Antibody.
Immunofluorescent analysis of Raji cells, using Bim Antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, IP, FC
Primary Accession	O43521
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Rat, Human, Mouse
Clonality	Monoclonal
Format	Liquid
Description	Anti-Bim BCL2L11 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Additional Information
Gene ID	10018
Other Names	Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BCL2L11, BIM
Calculated MW	22171 Da
Application Details	WB 1:500-1:1000 IHC 1:50-1:100 ICC/IF 1:50-1:100 IP 1:40 FC 1:50
Subcellular Localization	Endomembrane system ; Peripheral membrane protein. Associated with intracytoplasmic membranes..
Tissue Specificity	Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney..
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: CBE-2
Immunogen	A synthesized peptide derived from human Bim
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	BCL2L11
Synonyms	BIM
Function	Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase- mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.
Cellular Location	Endomembrane system; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes. [Isoform BimL]: Mitochondrion. [Isoform Bim-alpha1]: Mitochondrion.
Tissue Location	Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are widely expressed with tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.