癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-LEF1 Rabbit Monoclonal Antibody
- 产品详情
- 实验流程
Application 
| WB, IHC, IF, ICC, IP, FC |
|---|---|
| Primary Accession | Q9UJU2 |
| Host | Rabbit |
| Isotype | Rabbit IgG |
| Reactivity | Rat, Human, Mouse |
| Clonality | Monoclonal |
| Format | Liquid |
| Description | Anti-LEF1 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. |
| Gene ID | 51176 |
|---|---|
| Other Names | Lymphoid enhancer-binding factor 1 {ECO:0000312|HGNC:HGNC:6551}, LEF-1, T cell-specific transcription factor 1-alpha, TCF1-alpha {ECO:0000312|HGNC:HGNC:6551}, LEF1 (HGNC:6551) |
| Calculated MW | 44201 Da |
| Application Details | WB 1:1000-1:2000 IHC 1:50-1:200 ICC/IF 1:100-1:500 IP 1:50 FC 1:50 |
| Subcellular Localization | Nucleus. Found in nuclear bodies upon PIASG binding.. |
| Tissue Specificity | Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.. |
| Source | Eukaryota |
| Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| Clone Names | Clone: CFG-12 |
| Immunogen | A synthesized peptide derived from human LEF1 |
| Purification | Affinity-chromatography |
| Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | LEF1 (HGNC:6551) |
|---|---|
| Function | Transcription factor that binds DNA in a sequence-specific manner (PubMed:2010090). Participates in the Wnt signaling pathway (By similarity). Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity). PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (PubMed:11266540). Regulates T-cell receptor alpha enhancer function (PubMed:19653274). Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity). May play a role in hair cell differentiation and follicle morphogenesis (By similarity). |
| Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267}. Note=Found in nuclear bodies upon PIASG binding. |
| Tissue Location | Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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