Anti-Sonic Hedgehog SHH Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Western blot analysis of Sonic Hedgehog in HepG2 cell lysate.
Immunohistochemical analysis of paraffin-embedded human endometrium, using Sonic Hedgehog Antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, FC
Primary Accession	Q15465
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Human
Clonality	Monoclonal
Format	Liquid
Description	Anti-Sonic Hedgehog SHH Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Additional Information
Gene ID	6469
Other Names	Sonic hedgehog protein, SHH, 3.1.-.-, HHG-1, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhNC, Sonic hedgehog protein N-product, ShhN, Shh N-terminal processed signaling domains, ShhNp, SHH (HGNC:10848)
Calculated MW	49607 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 FC 1:50
Subcellular Localization	Sonic hedgehog protein C-product: Secreted, extracellular space. The C-terminal peptide diffuses from the cell..
Tissue Specificity	Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: ABE-19
Immunogen	A synthesized peptide derived from human Sonic Hedgehog
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SHH {ECO:0000303\|PubMed:7590746, ECO:0000312\|HGNC:HGNC:10848}
Function	[Sonic hedgehog protein]: Precursor of sonic hedgehog, a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during development (PubMed:29954986, PubMed:29995851, PubMed:30139912, PubMed:31127104, PubMed:31548691). The C-terminal part of the precursor displays an autoproteolysis and a cholesterol transferase activity, resulting (1) in the cleavage of the full-length protein into two parts, Sonic hedgehog protein N-product and C-product (ShhN and ShhC, respectively) and (2) covalent attachment of a cholesterol moiety to the C-terminus of the newly generated ShhN (By similarity). Both autoproteolysis and a cholesterol transferase activities occur in the endoplasmic reticulum (By similarity). Following additional lipidation, ShhN acts as a morphogen, while ShhC is degraded in the endoplasmic reticulum (By similarity).
Cellular Location	[Sonic hedgehog protein]: Endoplasmic reticulum membrane. Golgi apparatus membrane. Note=Co-localizes with HHAT in the ER and Golgi membrane.