癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-Renilla Luciferase Rabbit Monoclonal Antibody
- 产品详情
- 实验流程
Application 
| WB, IF, ICC |
|---|---|
| Primary Accession | P27652 |
| Host | Rabbit |
| Isotype | Rabbit IgG |
| Reactivity | Renilla Luciferase |
| Clonality | Monoclonal |
| Format | Liquid |
| Description | Anti-Renilla Luciferase Rabbit Monoclonal Antibody . Tested in WB, ICC/IF applications. This antibody reacts with Renilla Luciferase. |
| Other Names | Coelenterazine h 2-monooxygenase, 1.13.12.5, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase, LUCI |
|---|---|
| Calculated MW | 36022 Da |
| Application Details | WB 1:500-1:2000 ICC/IF 1:50-1:200 |
| Source | Eukaryota |
| Contents | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. |
| Clone Names | Clone: DAC-18 |
| Immunogen | A synthesized peptide derived from Renilla Luciferase |
| Purification | Affinity-chromatography |
| Storage | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | LUCI |
|---|---|
| Function | Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
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