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>   首页   >   产品   >   重组兔单克隆抗体   >   重组兔单克隆抗体   >   Renilla Luciferase Antibody   

Renilla Luciferase Antibody

Rabbit mAb

     
  • 1 - Renilla Luciferase Antibody AP90435
    Western blot analysis of Renilla Luciferase expression in (1) Renilla Luciferase transfected 293 cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.
  • 2 - Renilla Luciferase Antibody AP90435
    Immunofluorescent analysis of 293 cells transfected with Renilla Luciferase, using Renilla Luciferase Antibody .
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, ICC
Primary Accession P27652
Clonality Monoclonal
Other Names Renilla-type luciferase; Renilla luciferin 2 monooxygenase;
Isotype Rabbit IgG
Host Rabbit
Calculated MW 36022 Da
Additional Information
Dilution WB 1:500~1:2000 ICC/IF 1:50~1:200
Purification Affinity-chromatography
Immunogen A synthesized peptide derived from Renilla Luciferase
Description Renilla luciferin + O2 = oxidized Renilla luciferin + CO2 + light. The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly.
Storage Condition and Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name LUCI
Function Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.
Research Areas
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