Anti-p16 INK Monoclonal Antibody

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Western blot analysis of p16 INK expression in 293T cell lysate.
Immunohistochemical analysis of paraffin-embedded human gastric, using p16 INK Antibody.
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:3K dilution)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC, IP, FC
Primary Accession	P42771
Host	Rabbit
Isotype	Rabbit IgG
Reactivity	Human
Clonality	Monoclonal
Format	Liquid
Description	Anti-p16 INK Monoclonal Antibody . Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Additional Information
Gene ID	1029
Other Names	Cyclin-dependent kinase inhibitor 2A {ECO:0000312\|HGNC:HGNC:1787}, Cyclin-dependent kinase 4 inhibitor A, CDK4I, Multiple tumor suppressor 1, MTS-1, p16-INK4a, p16-INK4, p16INK4A, CDKN2A (HGNC:1787), CDKN2, MTS1
Calculated MW	16533 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 IP 1:30 FC 1:100
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: CGD-3
Immunogen	A synthesized peptide derived from human p16 INK
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CDKN2A (HGNC:1787)
Synonyms	CDKN2, MTS1
Function	Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
Cellular Location	Cytoplasm. Nucleus
Tissue Location	Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.