Anti-Adenylate kinase 4 Rabbit Monoclonal Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF, ICC
Primary Accession	P27144
Host	Rabbit
Isotype	IgG
Reactivity	Human
Clonality	Monoclonal
Format	Liquid
Description	Anti-Adenylate kinase 4 Rabbit Monoclonal Antibody . Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Additional Information
Gene ID	205
Other Names	Adenylate kinase 4, mitochondrial {ECO:0000255\|HAMAP-Rule:MF_03170}, AK 4 {ECO:0000255\|HAMAP-Rule:MF_03170}, 2.7.4.10 {ECO:0000255\|HAMAP-Rule:MF_03170, ECO:0000269\|PubMed:19766732, ECO:0000269\|PubMed:23416111}, 2.7.4.6 {ECO:0000255\|HAMAP-Rule:MF_03170, ECO:0000269\|PubMed:19766732, ECO:0000269\|PubMed:23416111}, Adenylate kinase 3-like {ECO:0000255\|HAMAP-Rule:MF_03170}, GTP:AMP phosphotransferase AK4 {ECO:0000255\|HAMAP-Rule:MF_03170}, AK4 {ECO:0000255\|HAMAP-Rule:MF_03170}
Calculated MW	25268 Da
Application Details	WB 1:500-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200
Source	Eukaryota
Contents	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clone Names	Clone: 27A39
Immunogen	A synthesized peptide derived from human Adenylate kinase 4
Purification	Affinity-chromatography
Storage	Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	AK4 (HGNC:363)
Function	Broad-specificity mitochondrial nucleoside phosphate kinase involved in cellular nucleotide homeostasis by catalyzing nucleoside- phosphate interconversions (PubMed:19073142, PubMed:19766732, PubMed:23416111, PubMed:24767988). Similar to other adenylate kinases, preferentially catalyzes the phosphorylation of the nucleoside monophosphate AMP with ATP as phosphate donor to produce ADP (PubMed:19766732). Phosphorylates only AMP when using GTP as phosphate donor (PubMed:19766732). In vitro, can also catalyze the phosphorylation of CMP, dAMP and dCMP and use GTP as an alternate phosphate donor (PubMed:19766732, PubMed:23416111). Moreover, exhibits a diphosphate kinase activity, producing ATP, CTP, GTP, UTP, TTP, dATP, dCTP and dGTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donors (PubMed:23416111). Plays a role in controlling cellular ATP levels by regulating phosphorylation and activation of the energy sensor protein kinase AMPK (PubMed:24767988, PubMed:26980435). Plays a protective role in the cellular response to oxidative stress (PubMed:19130895, PubMed:23474458, PubMed:26980435).
Cellular Location	Mitochondrion matrix {ECO:0000255\|HAMAP- Rule:MF_03170, ECO:0000269\|PubMed:11485571, ECO:0000269\|PubMed:19766732, ECO:0000269\|PubMed:26980435}
Tissue Location	Highly expressed in kidney, moderately expressed in heart and liver and weakly expressed in brain