Phospho-AP-1 (Ser63) Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Primary Accession	P05412
Reactivity	Human, Rat
Host	Rabbit
Isotype	Rabbit IgG
Calculated MW	35676 Da

Additional Information
Gene ID	3725
Positive Control	WB: HeLa cell lysate
Application & Usage	WB 1:500-1:2000; IHC 1:100-1:300; E 1:20000
Other Names	JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39
Target/Specificity	JUN
Antibody Form	Liquid
Appearance	Colorless liquid
Handling	The antibody solution should be gently mixed before use
Reconstitution & Storage	-20°C
Background Descriptions
Precautions	Phospho-AP-1 (Ser63) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	JUN
Function	Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' (PubMed:10995748, PubMed:22083952). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to the AP-1 consensus sequence 5'-TGA[GC]TCA-3' and enhancing its transcriptional activity (By similarity). Together with FOSB, plays a role in activation-induced cell death of T cells by binding to the AP-1 promoter site of FASLG/CD95L, and inducing its transcription in response to activation of the TCR/CD3 signaling pathway (PubMed:12618758). Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation (PubMed:17210646). Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
Cellular Location	Nucleus.
Tissue Location	Expressed in the developing and adult prostate and prostate cancer cells.