Phospho-c-Myc (Thr58) Antibody

Fig. A. WB analysis of NIH/3T3 cells using Phospho-c-Myc (T58) Polyclonal Antibody Fig. B. WB (WB) analysis of 293 cells using Phospho-c-Myc (T58) Polyclonal Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E, IP
Primary Accession	P01106
Reactivity	Human, Mouse, Rat
Host	Rabbit
Isotype	Rabbit IgG
Calculated MW	50565 Da

Additional Information
Gene ID	4609
Positive Control	WB: 3T3 and 293 cell lysate
Application & Usage	WB 1:500-1:2000; IHC 1:100-1:300; IP 1:200-1:500; E 1:10000
Other Names	Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64, MYC, BHLHE39
Target/Specificity	MYC
Antibody Form	Liquid
Appearance	Colorless liquid
Handling	The antibody solution should be gently mixed before use
Reconstitution & Storage	-20°C
Background Descriptions
Precautions	Phospho-c-Myc (Thr58) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	MYC
Synonyms	BHLHE39
Function	Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
Cellular Location	Nucleus, nucleoplasm. Nucleus, nucleolus. Nucleus. Cytoplasm Chromosome. Note=Association with chromatin is reduced by hyperphosphorylation (PubMed:30158517) Localization to the nucleolus is dependent on HEATR1 (PubMed:38225354)