Cyclin D1

Rabbit Monoclonal antibody(Mab)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human tonsil tissue was performed with Cyclin D1 primary antibody, followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human tonsil tissue was performed with Cyclin D1 primary antibody, followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human tonsil tissue was performed with Cyclin D1 primary antibody, followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human tonsil tissue was performed with Cyclin D1 primary antibody, followed by peroxidase-conjugated secondary antibody and DAB staining.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P
Primary Accession	P24385
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Clone Names	332G4S4
Calculated MW	33729 Da

Additional Information
Gene ID	595
Gene Name	CCND1
Other Names	G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene, CCND1 {ECO:0000303\|PubMed:8204893, ECO:0000312\|HGNC:HGNC:1582}
Dilution	IHC-P~~Pre-diluted Ready-To-Use (RTU)
Storage	Maintain refrigerated at 2-8°C.
Precautions	Cyclin D1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CCND1 {ECO:0000303\|PubMed:8204893, ECO:0000312\|HGNC:HGNC:1582}
Function	Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed:1827756, PubMed:1833066, PubMed:19412162, PubMed:33854235, PubMed:8114739, PubMed:8302605). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed:1827756, PubMed:1833066, PubMed:19412162, PubMed:8114739, PubMed:8302605). Hypophosphorylates RB1 in early G(1) phase (PubMed:1827756, PubMed:1833066, PubMed:19412162, PubMed:8114739, PubMed:8302605). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed:1827756, PubMed:1833066, PubMed:19412162, PubMed:8302605). Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity (PubMed:15241418). Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed:9106657). Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (PubMed:16569215, PubMed:18417529).
Cellular Location	Nucleus. Cytoplasm. Nucleus membrane. Note=Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members