Ywhae antibody - C-terminal region

Rabbit Polyclonal Antibody

Ywhae antibody - C-terminal region (AI139) validated by WB using Mouse Kidney lysate at 1.ug/ml.
Sample Type: 2. mouse brain extracts (8ug)
3. rat brain extract (8ug)
Primary Antibody Dilution: 2ug/ml
Secondary Antibody: IRDye 8CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution: 1: 2,
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical Science
Sample Type :
Human brain stem cells
Primary Antibody Dilution :
1:5
Secondary Antibody :
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution :
1:1
Color/Signal Descriptions :
Ywhae: Red DAPI:Blue
Gene Name :
Ywhae
Submitted by :
Dr. Yuzhi Chen, University of Arkansas for Medical Science

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P62259
Other Accession	NM_009536, NP_033562
Reactivity	Human, Mouse, Rat, Zebrafish, Bovine
Predicted	Mouse, Zebrafish, Chicken, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	29174 Da

Additional Information
Gene ID	22627
Alias Symbol	AU019196
Other Names	14-3-3 protein epsilon, 14-3-3E, Ywhae
Format	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution & Storage	Add 50 ul of distilled water. Final anti-Ywhae antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles.
Precautions	Ywhae antibody - C-terminal region is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	Ywhae
Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Positively regulates phosphorylated protein HSF1 nuclear export to the cytoplasm.
Cellular Location	Nucleus {ECO:0000250\|UniProtKB:P62258}. Cytoplasm {ECO:0000250\|UniProtKB:P62258}. Melanosome {ECO:0000250\|UniProtKB:P62258}

Research Areas