癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
GPR161 antibody - N-terminal region
Rabbit Polyclonal Antibody
- 产品详情
- 实验流程
Application 
| WB, IHC |
|---|---|
| Primary Accession | Q8N6U8 |
| Other Accession | NM_153832, NP_722561 |
| Reactivity | Human, Mouse, Rat, Rabbit, Pig, Dog, Horse |
| Predicted | Human, Mouse, Rabbit, Pig, Dog, Horse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 58559 Da |
| Gene ID | 23432 |
|---|---|
| Alias Symbol | FLJ33952, RE2 |
| Other Names | G-protein coupled receptor 161, G-protein coupled receptor RE2, GPR161 |
| Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reconstitution & Storage | Add 100 ul of distilled water. Final anti-GPR161 antibody concentration is 1 mg/ml in PBS buffer with 2% sucrose. For longer periods of storage, store at 20°C. Avoid repeat freeze-thaw cycles. |
| Precautions | GPR161 antibody - N-terminal region is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | GPR161 {ECO:0000303|PubMed:31609649, ECO:0000312|HGNC:HGNC:23694} |
|---|---|
| Function | G protein-coupled receptor that acts as a key inhibitor of the smoothened signaling pathway in primary cilia (PubMed:38326651). Inhibits the smoothened signaling pathway in absence of hedgehog (DHH, IHH or SHH) by promoting formation of the transcription repressor form of GLI2 and GLI3 transcription factors (PubMed:38326651). GPR161 activation triggers signaling via guanine nucleotide-binding protein G(s)/GNAS, mediating activation of adenylate cyclase activity and production of cAMP (PubMed:38326651). Increased cAMP levels activate PKA, promoting PKA-dependent processing of GLI2 and GLI3 transcription factors, leading to formation of transcription repressor GLI2R and GLI3R, which repress expression of target genes (PubMed:38326651). GPR161 also activates PKA by acting as a PKA-anchoring protein, which directly associates with type I PKA regulatory subunits (PRKAR1A and/or PRKAR1B), recruiting PKA followed by PKA-dependent processing of GLI2 and GLI3 transcription factors (PubMed:38326651). In presence of hedgehog, GPR161 is removed from primary cilia by smoothened (SMO), preventing its activity and allowing activation of the smoothened signaling (By similarity). In contrast to the majority of G protein- coupled receptors, it is not activated by a ligand and it either acts as a constitutively active receptor or as a mechanosensitive receptor (PubMed:38326651). Involved in regulation of the smoothened signaling pathway during early neural tube development, forelimb formation, skeletal development, embryonic cerebellar development and forebrain development (By similarity). Involved in the saltatory migration of neurons during brain development by acting as a mechanosensitive receptor that senses mechanical forces generated by fluid shear stress (By similarity). |
| Cellular Location | Cell projection, cilium membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Recruited to primary cilium in a TULP3 and IFT-A complex-dependent manner (PubMed:20889716). In presence of smoothened (SMO), it is removed from primary cilia and is internalized into recycling endosomes and is apparently not degraded (PubMed:40384633). Export from primary cilia is mediated via a beta-arrestin, BBSome and IFT-B complex-dependent mechanism (PubMed:40384633). |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
REFERENCES
Small,K.M., FEBS Lett. 516 (1-3), 253-256 (2002)Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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